Washington University in St Louis

The Preston M. Green Department of
Electrical & Systems Engineering

We build novel imaging technologies

Lew Lab group photo

The Lew Lab builds advanced imaging systems to study biological and chemical systems at the nanoscale. Our technology leverages innovations in applied optics, signal and image processing, design optimization, and physical chemistry. We partner with scientists and engineers across all disciplines to develop technologies to solve unmet needs in science, medicine, and society.

Diversity fosters creative ideas to challenging problems

Lew Lab diversity and inclusion
Poster courtesy of sammykatta.com/diversity.

The Lew Lab is a team of inventors, thinkers, and problem solvers working at the intersection of science and technology.

Creating impactful technology is our passion

We invent advanced nanoscopes with nanometer resolution that visualize single molecules in biological and chemical systems. [WebM - 48 MB]
Our novel microscopes robustly measure the 3D positions and 3D orientations of single fluorescent molecules using very little light. [WEBM - 13.0 MB]
Deep learning helps us robustly image molecules' positions and orientations using only 2D camera images with incredible speed and accuracy. [MP4 - 3.97 MB]
Our imaging systems resolve the nanoscale architecture of amyloid peptide aggregates, a key signature of Alzheimer's disease. [WebM - 20.3 MB]

Lab news

Jasmine Cheng's photo Jasmine was recognized at the 2024 McKelvey Engineers' Honors Ceremony with the David H. Levy Outstanding Senior Award. Congratulations!

Resolving the Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization MicroscopyWeiyan's extensive study on using orientation imaging to reveal the architectures of peptide self-assemblies is now online in ACS Nano!
Read it here: “Resolving the Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization Microscopy
Single-molecule orientation–localization microscopy captures fluorescence from Nile red molecules as they transiently bind to self-assembled fibrils composed of engineered KFE8 peptides. (Image credit: Weiyan Zhou)- Update: Weiyan's article is featured as a cover of Volume 18, Issue 12 of ACS Nano!

Dipole-Spread Function Engineering for Six-Dimensional Super-Resolution MicroscopyTingting's review chapter on dipole-spread function engineering for six-dimensional imaging, part of a new book, Coded Optical Imaging, is now online!
Read it here: “Dipole-Spread Function Engineering for Six-Dimensional Super-Resolution Microscopy

Congratulations to our close colleague Dr. Matt King in the Pappu lab on his study of pH gradients within the nucleolus, now appearing in Cell!
- Read it here: “Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient
- News coverage: The Source - Washington University, EurekAlert!

APS logoMatt gave an invited talk in the “Quantum Sensing and Information Processing for Biomedical Applications” session at the APS March Meeting in Minneapolis, entitled “6D Single-Molecule Orientation-Localization Microscopy: Fundamental Limits for Visualizing the Dynamic Organization of Biomolecules.”

More news...