Welcome to the Lew Lab archives! Here you'll find noteworthy milestones and past lab news. See how far the lab has come.
2024
Sam presented his summer research at the fall 2024 Undergraduate
Research Symposium!
Congratulations to Ezra and colleagues in the
Lee Lab at the
University of Cambridge! Their work on simple and computationally
efficient molecular orientation microscopy is now online in
Nature Methods! It was a saga getting this work completed.
Thank you, Oumeng, for demonstrating the accuracy of
RoSE-O in analyzing these data!
- Read it here: “POLCAM: instant molecular orientation microscopy for the life
sciences”
Weiyan's study of how noise-induced errors limit the accuracy of
measuring how molecules “wobble” in 3 dimensions is
now online in J. Phys. Chem. A!
- Read it here: “Fundamental Limits in Measuring the Anisotropic Rotational
Diffusion of Single Molecules”
- Our work is covered in McKelvey Engineering News: “Wobbly molecules get a closer look”
- Update: Weiyan's article is
featured as a cover
of Volume 128, Issue 28 of J. Phys. Chem. A!
Brian's study on using the binding orientations of Nile blue to
decipher the architectures of growing, decaying, and remodeling
amyloid-beta fibrils is now online in Nano Lett.!
- Read it here: “Single-Molecule Orientation Imaging Reveals the
Nano-Architecture of Amyloid Fibrils Undergoing Growth and
Decay”
- Read the accompanying news article, “WashU researchers shine light on amyloid architecture,” in The Source!
The lab welcomes Erik Lindeman, who received a Caltech Summer
Undergraduate Research Fellowship to visit us for a summer
project. We're excited to have you!
The lab welcomes Sam Kang, who has received a 2024 Summer
Undergraduate Research Award to work with us. Congratulations!
The Lew Lab welcomed Tingting back to campus for the
first-ever McKelvey PhD Hooding Ceremony. She also received an Outstanding Dissertation Award from the
Imaging Science Doctoral Program. Congratulations, Tingting!
The lab welcomes Suraj Deepak Khochare, who is pursuing a PhD in
Imaging Science.
Jasmine was recognized at the
2024 McKelvey Engineers' Honors Ceremony
with the David H. Levy Outstanding Senior Award. Congratulations!
Weiyan's extensive study on using orientation imaging to reveal
the architectures of peptide self-assemblies is now online in
ACS Nano!
- Read it here: “Resolving the Nanoscale Structure of β-Sheet Peptide
Self-Assemblies Using Single-Molecule Orientation–Localization
Microscopy”
- “Imaging technique shows new details of peptide structures” - The Source - Washington University
- Update: Weiyan's article is
featured as a cover
of Volume 18, Issue 12 of ACS Nano!
Tingting's review chapter on dipole-spread function engineering
for six-dimensional imaging, part of a new book,
Coded Optical Imaging, is now online!
Read it here: “Dipole-Spread Function Engineering for Six-Dimensional
Super-Resolution Microscopy”
Congratulations to our close colleague Dr. Matt King in the
Pappu lab on his study
of pH gradients within the nucleolus, now appearing in
Cell!
- Read it here: “Macromolecular condensation organizes nucleolar sub-phases to
set up a pH gradient”
- News coverage:
The Source - Washington University,
EurekAlert!
Matt gave an invited talk in the “Quantum Sensing and Information Processing for Biomedical
Applications” session at the APS March Meeting in Minneapolis, entitled
“6D Single-Molecule Orientation-Localization Microscopy:
Fundamental Limits for Visualizing the Dynamic Organization of
Biomolecules.”
Congratulations to Prof. Jin Lu,
now at the National Center for Nanoscience, whose work on single-molecule electrochemical imaging is now
online in Chemical Science!
- Read it here: “Single-Molecule Electrochemical Imaging Resolves the Midpoint
Potentials of Individual Fluorophores on Nanoporous
Antimony-Doped Tin Oxide”
- Update: Jin's article is now
featured as an inside cover
in Volume 15, Issue 6 of Chem. Sci.!
Matt gave an invited talk, “6D single-fluorogen orientation-localization microscopy for
elucidating the architecture of beta-sheet assemblies and
biomolecular condensates,” in the “High-Speed Biomedical Imaging and
Spectroscopy IX” conference at Photonics West in San
Francisco. [Open Scholarship,
Article]
Oumeng's news article, “Single-molecule orientation-localization microscopy,” is now online in Imaging & Microscopy.
2023
Tingting successfully defended her thesis,
“Information-Rich 6D Single-Molecule Imaging Using
Optimization,” on Monday, November 20, at 1 PM in Green
Hall's Rodin Auditorium. Congratulations and we will miss you,
Tingting!
We hiked together and celebrated Tingting's many contributions to
the group. After graduating, she will be a Senior Optical
Metrology Engineer at Metalenz in Boston, MA. Thank you for
bringing advanced computation and biomolecular condensates to the
lab!
Brian and Jasmine presented their research at the 2nd annual
SPECTRA student-led
conference. Both the talks and poster session had superb
attendance. Thanks to the wonderful 2023 officers for bringing
together the WashU optics community!
Matt presented “Single-fluorogen imaging reveals the
nanoscale structure of beta-sheet assemblies and biomolecular
condensates” in the
“Single-molecule Dynamics in Complex Chemical and
Biological Systems” session
at SERMACS 2023.
“Capturing images of molecules with a new level of resolution: An interview with Prof. Matthew Lew” was published in Wiley Analytical Science magazine.
Congratulations to Weiyan, who won a poster award at the Chemical
Imaging GRC 2023!
Our lab has received a five-year $2 million Maximizing Investigators’ Research Award (MIRA) from the National Institutes of Health (NIH) to develop adaptive six-dimensional microscopy. Congrats, all!
Congratulations, Tingting, on receiving a 2023 Outstanding
Research Award from the Imaging Science Doctoral Program!
Congratulations, Jasmine, on receiving a 2023 Summer Undergraduate Research Award!
Two outstanding undergraduates have received awards this year:
Brian with the Rick Grodsky Award for Technical Achievement and
the David H. Levy Outstanding Senior Award, and Jasmine with the
Russel R. Pfeiffer Outstanding Junior Award! Both were recognized
at the
2023 McKelvey Engineers' Honors Ceremony. Congratulations, Brian and Jasmine!
Matt has received a
2023 Outstanding Faculty Award
from the
Graduate Student Senate. The awardees were
recognized at a ceremony
organized by the GSS. Thank you, students, for this honor!
The Lew Lab presented several exciting posters and talks at
BPS 2023 in San Diego:
- Congratulations to Tingting, who has received a 2023 Travel
Award to present at the Annual Meeting!
- Congratulations to Weiyan, whose submission to the Biophysical
Society Art of Science Image Contest was selected as one of ten
finalists! Her art was displayed in the Exhibit Hall during the
meeting.
- Weiyan presented “Single-Molecule Orientation-Localization
Microscopy Resolves the Nanoscale Organization of Self-Assembled
Peptides,” Poster 627-Pos / B448 in Optical Microscopy and
Superresolution Imaging I on Sunday, Feb 19 [Abstract]
- Brian presented “Single-Molecule Orientation-Localization
Microscopy Resolves How Amyloidophilic Dye Orientations are
Correlated with Amyloid Fiber Growth and Disruption,” Poster
1339-Pos / B468 in Optical Microscopy and Superresolution Imaging
II on Monday, Feb 20 [Abstract]
- Matt presented “A Multi-View Reflector Microscope
Visualizes Six-Dimensional Single-Molecule Translational And
Rotational Dynamics With Isotropic Nanoscale Resolution,”
Poster 1351-Pos / B480 in Optical Microscopy and Superresolution
Imaging II on Monday, Feb 20 [Abstract]
- Tingting presented “Mapping Inhomogeneous Network
Structures within Biomolecular Condensate using Single-Molecule
Imaging and Tracking of Fluorogenic Probes,”
Platform Talk 1437-Plat in Condensates: Physical
Properties and Modeling II on
Tuesday, Feb 21 at 9:15 AM [Abstract]
2022
Congratulations to Oumeng and our collaborators in the
Vahey Lab in WashU
Biomedical Engineering on publishing a new microscope architecture
for 6D single-molecule super-resolution imaging in
Nature Photonics! The multi-view reflector (MVR)
microscope achieves nearly isotropic 10.9 nm precision in 3D over
a 1.5 μm depth range and measures the 3D orientation of single
molecules with 2.0° precision.
- “Six-dimensional single-molecule imaging with isotropic
resolution using a multi-view reflector microscope,”
Nat. Photon. 17, 179 (2023). [The Source - Washington University,
Open Scholarship,
Article, Data,
Summary PDF]
- “Telescope-inspired microscope sees molecules in 6D” in the Source.
Tingting's collaborative work with Peng and Asheq on using
deep-learning for 5D imaging (2D position and 3D orientation
estimation) of single molecules is now online in
Optics Express. Congratulations!
- “Deep-SMOLM: deep learning resolves the 3D orientations
and 2D positions of overlapping single molecules with optimal
nanoscale resolution,” Opt. Express 30, 36761
(2022). [McKelvey Engineering News,
Article,
Code, Data,
Video abstract]
- “Machine learning generates pictures of proteins in 5D” in McKelvey Engineering News.
Matt led the “Single Molecules and Phase” session at
the 65th Welch Conference on Chemical Research, “Molecules
and Sculpted Light.”
W. E. Moerner chaired the conference, which featured awesome science and timely addresses from Peter
Hotez and Carolyn R. Bertozzi.
The Lew Lab is thrilled to be part of the new
Center for Biomolecular Condensates
in the McKelvey School of Engineering. The
inaugural symposium
was inspirational and great fun! [McKelvey Engineering News]
James's theoretical exploration of how to design microscopes
optimized for resolving closely spaced objects in 3D is now online
in Optics Express. Congratulations!
- “Towards optimal point spread function design for
resolving closely spaced emitters in three dimensions,”
Opt. Express 30, 37154 (2022). [Article]
- James's paper has been highlighted as an
Editor's Pick!
Dr. Meikun Shen, formerly in the Sadtler group, has published a
study on how ascorbic acid enhances water oxidation activity on
the surfaces of tungsten oxide nanowires. It appears in
Nano Lett. Congratulations!
“In Situ Imaging of Catalytic Reactions on Tungsten
Oxide Nanowires Connects Surface–Ligand Redox Chemistry with
Photocatalytic Activity” [Article]
Matt is spotlighted as a STEM expert in the summer 2022 issue of
the Saint Louis Science Center's NewScience magazine. He
discusses the incredible potential of computational imaging in
“Can a Computer Help Make a Better Camera?”.
Read:
Magazine version,
Web/mobile version,
PDF [51.8 MB]
Congratulations, Oumeng, on being featured as an outstanding graduate by the McKelvey School of Engineering!
Congratulations, Jasmine, on receiving a 2022 Summer Undergraduate
Research Award!
Tingting's work on engineering new dipole-spread functions (DSFs)
for imaging single molecules in 6 dimensions (3D position plus 3D
orientation) is now online in Optica. Congratulations!
- “Dipole-spread-function engineering for simultaneously
measuring the 3D orientations and 3D positions of fluorescent
molecules,” Optica 9, 505 (2022). [Article, Data,
Video abstract]
-
“Lew lab sheds new light on cell membranes” - The
Source
The lab welcomes Yiyang Chen and Yuanxin Qiu, both in the Imaging
Science PhD program. It's great to have you!
Matt give another invited colloquium, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the Department of Physics & Astronomy at San Francisco State University.
Matt give an invited talk, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the MIT Laser Biomedical Research Center's Modern Optics and Spectroscopy Seminar.
Congratulations to Dr. Hesam Mazidi, whose patent on using Wasserstein-induced flux to certify localization accuracy has been granted!
Tingting presented “pixOL: pixel-wise dipole-spread function engineering for simultaneously measuring the 3D orientation and 3D location of dipole-like emitters” at Focus on Microscopy 2022.
Congratulations, Dr. Oumeng Zhang, on
successfully defending his PhD dissertation!
We congratulate Oumeng on receiving the Department of Electrical and Systems Engineering's Outstanding Doctoral Dissertation Award! Thank you for your outstanding contributions to the technology and culture of innovation in our lab!
We also congratulate Brian on receiving the Russell R. Pfeiffer Outstanding Junior Award! It is well-deserved!
The Washington University in St. Louis Board of Trustees has promoted Matt to the rank of Associate Professor with tenure. Onward and upward!
Congratulations Tingting on receiving a second-place poster award
at the
2022 Imaging Sciences Pathway Retreat!
The Lew Lab presented several posters at
BPS 2022 in San Francisco:
- “Accurate Superresolution Microscopy of the 3D
Orientations and 3D Locations of Single Molecules within Lipid
Membranes and Biomolecular Condensates,” Poster 694-Pos /
B518 in Optical Microscopy and Superresolution Imaging I [Abstract]
- “Visualizing the Nanoscale Architecture of Amyloid
Aggregates using Amyloidophilic Probes and a Polarized Vortex
Microscope,” Poster 693-Pos / B517 in Optical Microscopy and
Superresolution Imaging I [Abstract]
- “Mapping the Chemical Composition and Nanoscale Structure
of Lipid Membranes using Polarized Fluorescence from Single
Molecules,” Poster 2353-Pos / B183 in Membrane Structure II
[Abstract]
Today, the Lew Lab celebrates the science and mentorship of
W. E. Moerner
and the
publication of a Festschrift issue
of the Journal of Physical Chemistry dedicated to him.
Thank you, W. E., for your pioneering work and leadership that
continues to inspire generations of scientists and engineers!
Read:
-
Tribute to W. E. Moerner
-
Autobiography of W. E. Moerner
-
About the cover
- Our contribution to the Festschrift: “Single-Molecule Localization Microscopy of 3D Orientation and
Anisotropic Wobble Using a Polarized Vortex Point Spread
Function”
Welcome, Wentao, to the Lew Lab!
Oumeng's study of using radially and azimuthally polarized
fluorescence to measure the translational and rotational dynamics
of Nile red in lipid bilayers is now online in
Nano Letters. Congratulations!
- “Resolving the Three-Dimensional Rotational and
Translational Dynamics of Single Molecules Using Radially and
Azimuthally Polarized Fluorescence,” Nano Lett.
22, 1024 (2022). [Open Scholarship,
Article, Summary PDF]
-
“Lew lab sheds new light on cell membranes” - The
Source
2021
Tianben's postdoctoral work on developing the polarized vortex PSF
for imaging the 3D orientations and 3D wobble of single molecules
is now online in J. Phys. Chem. B. Congratulations!
“Single-Molecule Localization Microscopy of 3D Orientation
and Anisotropic Wobble Using a Polarized Vortex Point Spread
Function,” J. Phys. Chem. B 125, 12718 (2021).
[The Source - Washington University,
EurekAlert!,
Open Scholarship, Article,
Summary PDF
Update: Tianben's art has been selected as a supplemental cover for volume 125, issue 46 of J. Phys. Chem. B!
Matt gave a talk about single-molecule imaging of electron
transfer pathways at
NA-ISMET 2021
in Los Angeles, CA. There were many fascinating talks covering all
aspects of microbial electrochemistry and technology. Thanks to
Moh and his group for
organizing and hosting!
Matt gave an invited talk in the “Single-Molecule Approaches
to Chemistry and Biology” session at
SERMACS 2021 in
Birmingham, AL. It was great to see such fantastic single-molecule
studies in person! Thanks
Kevin for organizing!
We got together for a beautiful hike and celebrated Tianben's many
contributions to the group. We wish him well as a new researcher
at Hitachi, Ltd. in Japan. Thank you for inspiring us with new
optical technologies and studies on catalysis and amyloid imaging!
Tianben and Tingting gave platform presentations at the
Microscopy and Microanalysis 2021 Virtual Meeting, Symposium B11.3 -
Frontiers in Fluorescence Lifetime and Super-resolution Imaging
of Biological Structures and Dynamics.
Congratulations, Tingting, on being designated a
2021 M&M Student Scholar!
Dr. Meikun Shen, formerly in the Sadtler group, has published
another study on surface heterogeniety and charge-carrier
separation in semiconductor photocatalysts. It appears in
J. Am. Chem. Soc. Congratulations!
“Single-Molecule Colocalization of Redox Reactions on
Semiconductor Photocatalysts Connects Surface Heterogeneity and
Charge-Carrier Separation in Bismuth Oxybromide” [Article]
Matt was designated as an OSA Senior Member. Congratulations!
Matt presented at the
2021 OSA Imaging and Applied Optics Congress:
Matt spoke at the “Probing Chemical Reactions by Single-Molecule Spectroscopy” virtual conference. It was a fascinating day of
single-molecule research! Thank you
Suzanne and
Randy for
organizing!
The lab extends a (belated) welcome to Weiyan, Ziyi, and Makaiya!
Weiyan Zhou has joined our lab for her PhD studies. Ziyi Hu and
Makaiya Smith are working with us on summer research projects.
Learn more about them.
After more than a year apart, the group celebrated Hesam's and
Tianben's accomplishments. Congratulations on earning your
PhDs!
We also wished Jin farewell as he begins his new position at the
National Center for Nanoscience and Technology in Beijing. Thank
you for making us all better chemists!
Congratulations to Tingting on receiving the department's
Outstanding Graduate Student Assistant to the Instructor (AI)
Award! Tingting received this award during the
McKelvey Engineering Honor Ceremony
held on April 21, 2021.
Oumeng and Matt presented talks at
Focus on Microscopy 2021 online, March 28-31:
- “Measuring Single-Molecule Orientations Using a Radially
and Azimuthally Polarized Epifluorescence Microscope”
- “Visualizing Membrane Composition and Amyloid Aggregate
Organization Using Single-Molecule 3D Orientation Imaging”
The lab welcomes Brian Sun, who has received a Summer Undergraduate Research Award to work with us this summer on amyloid imaging. Congratulations Brian!
Oumeng's two-part series exploring orientation-localization
microscopy has been accepted to
J. Opt. Soc. A!
- “Single-molecule orientation localization microscopy I:
fundamental limits” [arXiv.org,
Article]
- “Single-molecule orientation localization microscopy II: a
performance comparison” [arXiv.org,
Article]
And, the SMOLM II paper has been selected as an
“Editors' Pick”! Congratulations, Oumeng!
Matt gave an invited seminar “Toward Visualizing the Dynamic
Organization of Amyloid Aggregates using Single-Molecule
Orientation Localization Microscopy” at the
Knight Alzheimer Disease Research Center
at Washington University.
2020
Hesam's development and characterization of Wasserstein Induced
Flux for certifying the statistical confidence of localization
microscopy datasets is now online in
Nature Communications! Congratulations, Hesam!
Read the press coverage:
The Source - Washington University,
EurekAlert!
Read the article:
“Quantifying accuracy and heterogeneity in
single-molecule super-resolution microscopy”
Matt has been honored with the 2020 “Excellence in Teaching” award from Emerson Electric Co.! Each year, honorees are chosen by the deans of their schools and recognized by the Center for Teaching and Learning.
Tianben, Jin, and Oumeng presented their recent work at
S3IC 2020: Single-Molecule Sensors and
NanoSystems. Check out their presentations here:
Congratulations to Oumeng, whose patent covering the tri-spot PSF has been granted!
Update: The paper has been selected by the editors as a
“Hot Paper” on Nanoscale Sensors. Congratulations,
Jin!
Read the press coverage:
The Source - Washington University,
The Analytical Scientist,
EurekAlert!
Read the article:
“Single-molecule 3D orientation imaging reveals nanoscale
compositional heterogeneity in lipid membranes”
, updated 8.3.20
Congratulations to Dr. Hesam Mazidi, Dr. Tianben Ding, and Jack Hyde on successfully defending their dissertations! It's been a pleasure having you in the lab!
Oumeng's work on deriving the quantum limits of measuring
molecular orientation is now online in
Phys. Rev. Research. Congratulations, Oumeng!
“Quantum limits for precisely estimating the orientation and
wobble of dipole emitters” [Article]
Meikun in the Sadtler group has published another study on the
photocatalytic activity of semiconductor nanoplates in
J. Phys. Chem. Lett. Congratulations!
“Competing Activation and Deactivation Mechanisms in
Photodoped Bismuth Oxybromide Nanoplates Probed by Single-Molecule
Fluorescence Imaging” [Article]
Tianben and Tingting's work on using super-resolution
single-molecule orientation localization microscopy to study
amyloid aggregates is now online in Optica.
Congratulations!
See:
single-molecule orientation localization microscopy in
action
Read the press coverage:
OSA news release,
The Source - Washington University,
EurekAlert!,
Photonics Media
Read the article:
“Single-molecule orientation localization microscopy for
resolving structural heterogeneities between amyloid
fibrils”
We welcome Tristan Carlson and James Cevasco to the lab!
Congratulations to Max on his Summer Undergraduate Research
Award! Max will be working with Jack on our ultrafast camera
collaboration with the Buckley lab in Physics.
Matt has been recognized with the 2019-2020 Outstanding Teaching Award from the Department of Electrical and Systems Engineering! Matt received the award as part of the ESE Awards and Recognition Ceremony on May 8, 2020.
Congratulations to Jack for winning the
Rick Grodsky ESE Award for Technical Achievement! Jack received the award as part of the
ESE Awards and Recognition Ceremony
on May 8, 2020.
Matt and Oumeng presented at the
Computational Optical Imaging workshop
at the IEEE ISBI 2020 virtual conference.
Our collaborative work on imaging photocatalytic activity,
jointly led by Tianben and Meikun in the Sadtler group in the
Department of Chemistry, is now online in ACS Catalysis.
Congratulations, all!
“Nanoscale Colocalization of Fluorogenic Probes Reveals Role
of Oxygen Vacancies in the Photocatalytic Activity of Tungsten
Oxide Nanowires” [Open Scholarship,
Article]
Press coverage:
The Source - Washington University
Jin and Tianben presented their recent work at the 64th Annual Meeting of the Biophysical Society in San Diego.
Hesam and Tingting presented their recent work in the “Single Molecule Spectroscopy and Superresolution Imaging
XIII” session at SPIE Photonics West in San Francisco. Read
about their work here:
“Measuring localization confidence for quantifying accuracy
and heterogeneity in single-molecule super-resolution
microscopy” [Open Scholarship, Article]
“A computationally-efficient bound for the variance of
measuring the orientation of single molecules” [Open Scholarship, Article]
Hesam's work on “Quantifying Localization Accuracy in
Single-Molecule Super-Resolution Microscopy” was presented
in the “New Frontiers in Single Molecule Microscopy”
session at
QBI 2020
in Oxford, UK.
2019
Matt gave an invited talk in the “Computational Modeling in
High-Resolution Imaging” session at the
25th Solvay Conference on Chemistry
“Computational Modeling: From Chemistry to Materials to
Biology” in Brussels, Belgium.
Oumeng's paper entitled “Fundamental limits on measuring the rotational constraint of
single molecules using fluorescence microscopy” has been published in Physical Review Letters.
Congratulations! [arXiv.org,
Article]
Press coverage:
The Source - Washington University
Oumeng's and Hesam's works were presented at the OSA
Imaging and Applied Optics Congress
on June 24-27, 2019, in Munich:
“Fundamental Limits on Imaging the Orientational Dynamics of
Dipole-Like Emitters”
“Single-Molecule Super-Resolution Imaging of Molecular
Orientation using a Tri-Spot Point Spread Function”
The lab welcomes James Jusuf and Tara Porter to St. Louis! Both
James and Tara were awarded Summer Undergraduate Research
Fellowships from Caltech to lead their own research projects in
our group.
Matt gave an invited talk at Washington University's
Imaging Sciences Pathway Retreat 2019
entitled “Single Molecules and Point Spread Function
Engineering for Visualizing Amyloid Protein Dynamics at the
Nanoscale.” The group also presented several posters at the
event!
Matt gave an invited talk at the
5th Annual Meeting of the Biophysical Society of Canada
at the University of Toronto, Mississauga.
The group threw a farewell board game party for Eshan, Kevin, and
Weiran. Thank you all for being wonderful colleagues, and we wish
you the best in your next endeavors!
Eshan presented his and Hesam's work on DNA imaging at the IEEE
International Symposium on Biomedical Imaging (ISBI)
on Apr. 8-11, 2019, in Venice.
“Dense Super-Resolution Imaging of Molecular Orientation Via
Joint Sparse Basis Deconvolution and Spatial Pooling” [arXiv.org,
Article]
Oumeng and Kevin presented their work at the OSA
Biophotonics Congress: Optics in the Life Sciences
meeting on Apr. 14-17, 2019, in Tuscon.
“Measuring Rotational Dynamics with High Accuracy and Precision
Using a Tri-spot Point Spread Function”
“Long-Term Super-Resolution Imaging of Amyloid Structures Using
Transient Binding of Thioflavin T” [OSA News Release]
Our lab has begun working with a multi-university team to build functional living electronics, called Livtronics, as part of the Office of Naval Research-funded Multidisciplinary University Research Initiative (MURI) Program. Our aim is to build new fluorescence imaging technologies to visualize electron transport within living systems. Read more about it here. [McKelvey School of Engineering news]
Tianben and Oumeng presented their work at Photonics West. See it
here:
“Long-term, super-resolution imaging of amyloid
structures using transient amyloid binding microscopy”
“Fundamental limits of measuring single-molecule
rotational mobility”
Tingting Wu, a PhD student in our Imaging Science PhD program,
has joined the Lew Lab. Welcome, Tingting!
2018
Jack presented his summer research project at WashU's Fall 2018
Undergraduate Research Symposium.
Kevin's and Tianben's article on super-resolution transient
amyloid binding (TAB) microscopy is a
featured cover article
at ChemBioChem. Congratulations to the entire team! Read
it here:
“Super-Resolution Imaging of Amyloid Structures over
Extended Times Using Transient Binding of Single Thioflavin T
Molecules”
[Open Scholarship]
Press coverage:
The Source - Washington University,
NSF Science360,
Futurity,
EurekAlert!
Henry Cohen, who is studying for an MS degree in Biomedical
Engineering, has joined the Lew Lab and received a Master's
Fellowship. Welcome, Henry!
Hesam's article on the characteristics of structural bias in
super-resolution microscopy and how to minimize it using our RoSE
algorithm is now online at Scientific Reports.
Congratulations! Read it here:
“Minimizing Structural Bias in Single-Molecule
Super-Resolution Microscopy”
Weiran Wang, who is studying for an MS degree in Electrical
Engineering, has joined the Lew Lab and received a Master's
Fellowship. Welcome, Weiran!
Our collaborative work with Dr. Greg Lanza on imaging the
intracellular fate of lipid prodrugs is a
featured cover article
in Precision Nanomedicine. Read it here:
“Cellular Trafficking of Sn-2 Phosphatidylcholine
Prodrugs Studied with Fluorescence Lifetime Imaging and
Super-resolution Microscopy”
Oumeng's article
on the Tri-spot point spread function is now online at
Applied Physics Letters. Congratulations Oumeng! Read it
here:
“Imaging the three-dimensional orientation and rotational
mobility of fluorescent emitters using the Tri-spot point spread
function”
[Open Scholarship]
Matt gave an invited Stanford Optical Society seminar entitled
“Computational Optics for Multidimensional Nanoscale Imaging of
Single Fluorescent Molecules.”
Jack Hyde, who is majoring in Electrical Engineering, has joined
the Lew Lab. He has received a Summer Undergraduate Research Award
from the Office of Undergraduate Research. Welcome, Jack!
Kevin Spehar, who is majoring in Biomedical Engineering, has
joined the Lew Lab. Welcome, Kevin!
Hesam and Oumeng gave excellent presentations at SPIE BiOS
(Photonics West). See them here:
“A robust statistical estimation (RoSE) algorithm jointly
recovers the 3D location and intensity of single molecules
accurately and precisely”
“Measuring 3D molecular orientation and rotational
mobility using a Tri-spot point spread function”
2017
Matt has received a five-year, $1.6 million Maximizing
Investigators' Research Award from the National Institute of
General Medical Sciences, part of the National Institutes of
Health. The project is entitled "Five-Dimensional Single-Molecule Nanoscopy for Sensing and
Imaging the Dynamic Functions of Biomolecules." We are collaborating with Jan Bieschke at UCL's
Institute of Prion Diseases. Congratulations team! [The Source - Washington University]
Eshan presented his summer research project at the WashU Fall
2017 Undergraduate Research Symposium.
Hesam's and Oumeng's abstracts have been accepted for oral presentations in the "Single Molecule Spectroscopy and Superresolution Imaging XI" conference at SPIE BiOS (Photonics West). We're looking forward to showcasing our work in San Francisco in 2018! [Conference program]
Eshan King, who is majoring in Biomedical Engineering and Applied
Mathematics, has joined the Lew Lab. He has received a Summer
Undergraduate Research Award from the Office of Undergraduate
Research. Welcome, Eshan!
We have received a Collaboration Initiation Grant from the School of Engineering & Applied Science to build a fluorescence-lifetime imaging nanoscope in collaboration with James Buckley in the Department of Physics. Congratulations team! [WashU Engineering News]
Oumeng has successfully defended his Master's thesis, "Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function." Congratulations Oumeng! [ESE MS Defense Announcement PDF]
Matt has received a five-year, $500,000 CAREER Award from the
National Science Foundation for the project entitled "CAREER: Nanoscale sensing and imaging using computational
single-molecule nanoscopy." Congratulations team! [WashU Engineering News,
The Source - Washington University]
2016
Dr. Jin Lu joined the Lew Lab. Welcome Jin!
Our joint project "Single-Molecule, Super-Resolution Imaging
of Reaction Sites in Mesoscale Semiconductor Photocatalysts"
with
Prof. Bryce Sadtler
in the Department of Chemistry
has been funded by the WashU International Center for Advanced
Renewable Energy and Sustainability (I-CARES)!
Matt was awarded the Hiruma/Wagner Award at the
16th Conference of Peace through Mind/Brain Science.
We imaged single fluorescent molecules for the first time! What
beautiful blinking!
2015
Tianben Ding, a PhD student in Electrical and Systems
Engineering, joined the Lew Lab. Welcome, Tianben!
Matt won first place in Optics and Photonics News's
10th annual After Image photo contest!
Matt gave an invited talk at Laser Science 2015 entitled “Accurate 3D nanoscale imaging of dipole-like emitters.”
Lab construction is completed and ready for equipment move-in!
Matt gave the first lecture of ESE 582/BME 5820, Fundamentals and Applications of Modern Optical Imaging, a new graduate course at Washington University.
Matt joined the the Department of Electrical and Systems Engineering at Washington University in St. Louis as an Assistant Professor.