Washington University in St Louis

The Preston M. Green Department of
Electrical & Systems Engineering

We build novel imaging technologies

Lew Lab group photo

The Lew Lab builds advanced imaging systems to study biological and chemical systems at the nanoscale. Our technology leverages innovations in applied optics, signal and image processing, design optimization, and physical chemistry. We partner with scientists and engineers across all disciplines to develop technologies to solve unmet needs in science, medicine, and society.

Lew Lab diversity and inclusion
Poster courtesy of sammykatta.com/diversity.

The Lew Lab is a team of inventors, thinkers, and problem solvers working at the intersection of science and technology. Excited about joining our team?

Creating impactful technology is our passion

Lighting up amyloids | Inside the Research | Washington University [WebM - 15.4 MB]
“Flyover” animation of single-molecule orientation localization microscopy [WebM - 20.3 MB]. See more animations and interactive demos.

We develop advanced nanoscopes (microscopes with nanometer resolution) that can visualize the activity of individual molecular machines inside and outside living cells. Examples of our technology include 1) using tiny fluorescent molecules as sensors that can detect amyloid proteins, 2) designing new “lenses” to create imaging systems that can visualize how molecules move and tumble, and 3) new imaging software that minimizes artifacts in super-resolution images.

Lab news

Dipole-spread-function engineering for simultaneously measuring the 3D orientations and 3D positions of fluorescent molecules Tingting's work on engineering new dipole-spread functions (DSFs) for imaging single molecules in 6 dimensions (3D position plus 3D orientation) is now online in Optica. Congratulations!
“Dipole-spread-function engineering for simultaneously measuring the 3D orientations and 3D positions of fluorescent molecules,” Optica 9, 505 (2022). [Article]

Matt give another invited colloquium, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the Department of Physics & Astronomy at San Francisco State University.

Matt give an invited talk, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the MIT Laser Biomedical Research Center's Modern Optics and Spectroscopy Seminar.

Congratulations to Dr. Hesam Mazidi, whose patent on using Wasserstein-induced flux to certify localization accuracy has been granted!

Tingting presented “pixOL: pixel-wise dipole-spread function engineering for simultaneously measuring the 3D orientation and 3D location of dipole-like emitters” at Focus on Microscopy 2022.

Oumeng successfully defends his PhD thesis on April 7, 2022 Congratulations, Dr. Oumeng Zhang, on successfully defending his PhD dissertation!

We congratulate Oumeng on receiving the Department of Electrical and Systems Engineering's Outstanding Doctoral Dissertation Award! Thank you for your outstanding contributions to the technology and culture of innovation in our lab!

We also congratulate Brian on receiving the Russell R. Pfeiffer Outstanding Junior Award! It is well-deserved!

The Washington University in St. Louis Board of Trustees has promoted Matt to the rank of Associate Professor with tenure. Onward and upward!

Tingting receives a second-place poster award at the 2022 WashU Imaging Sciences Pathway Retreat Congratulations Tingting on receiving a second-place poster award at the 2022 Imaging Sciences Pathway Retreat!

Tingting gives a flash talk at BPS 2022 in San Francisco, CA The Lew Lab presented several posters at BPS 2022 in San Francisco:
- “Accurate Superresolution Microscopy of the 3D Orientations and 3D Locations of Single Molecules within Lipid Membranes and Biomolecular Condensates,” Poster 694-Pos / B518 in Optical Microscopy and Superresolution Imaging I [Abstract]
- “Visualizing the Nanoscale Architecture of Amyloid Aggregates using Amyloidophilic Probes and a Polarized Vortex Microscope,” Poster 693-Pos / B517 in Optical Microscopy and Superresolution Imaging I [Abstract]
- “Mapping the Chemical Composition and Nanoscale Structure of Lipid Membranes using Polarized Fluorescence from Single Molecules,” Poster 2353-Pos / B183 in Membrane Structure II [Abstract]

More news...